MIQE

Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Stephen Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller,Tania Nolan, Michael Pfaffl, Gregory Shipley, Jo Vandesompele, Carl Wittwer

MIQE is a set of guidelines that describe the minimum information necessary for evaluation of quantitative real-time polymerase chain reaction experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher.  By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.  Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of quantitative PCR results.

Updated information hosted on RDML web site

SAB with Kary Mullis

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Stephen A Bustin BA(mod) PhD (TCD)

Professor of Molecular Science
Institute of Cell and Molecular Science
Barts and the London
Queen Mary's School of Medicine and Dentistry
University of London

Visiting Professor of Molecular Biology
School of Health and Social Sciences
Middlesex University

Centre for Academic Surgery, 3rd Floor Alexandra Wing, Royal London Hospital, London E1 1BB, England

Tel: +44-(0)20 7882 8748

Email: s.a.bustin@qmul.ac.uk

Last updated 01/06/2009