Peer-reviewed publications since 2000
Nolan T, Hands RE, Bustin SA
Quantification of mRNA using real-time RT-PCR Nature Protocols 2006 1:1559-1582
Beyan H, Goodier MR, Nawroly NS, Hawa MI, Bustin SA, Ogunkolade WB, Londei M, Yousaf N, Leslie RD.
Altered monocyte cyclooxygenase response to lipopolysaccharide in type 1 diabetes. Diabetes. 2006 55:3439-45.
PMID: 17130490
McVittie C, Khalaf S, Ogunkolade W, McCarthy K, Laban C, Macaulay VM,
Carpenter R, Bustin SA, Jenkins P.
Extended silencing of the insulin-like growth factor-I receptor gene in MCF-7
cells by serial transfection with siRNA.
Anal Biochem. 2006 357:305-07
PMID: 16942747
Ogunkolade WB, Boucher BJ, Bustin SA, Burrin JM, Noonan K, Mannan N, Hitman
GA.
Vitamin D Metabolism in Peripheral Blood Mononuclear Cells is influenced by
Chewing 'Betel nut' (Areca catechu) and Vitamin D Status.
J Clin Endocrinol Metab. 2006 91:2612-7
PMID: 1667016
McCarthy K, Bustin SA, Ogunkolade B, Khalaf S, Laban CA, McVittie CJ,
Carpenter R, Jenkins PJ.
Cyclo-oxygenase-2 (COX-2) mRNA expression and hormone receptor status in breast
cancer.
Eur J Surg Oncol. 2006 32:707-09
PMID: 16650963
Nolan T, Hands RE, Ogunkolade W, Bustin SA.
SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid
preparations.
Anal Biochem. 2006 Apr 15;351(2):308-10.
PMID: 16524557
Bustin SA, Mueller R.
Real-time reverse transcription PCR and the detection of occult disease in
colorectal cancer.
Mol Aspects Med. 2006 Apr-Jun;27(2-3):192-223. Epub 2006 Jan 30.
PMID: 16445974 [PubMed - in process]
Jenkins PJ, Khalaf S, Ogunkolade W, McCarthy K, David T, Hands RE, Davies D,
Bustin SA.
Differential expression of IGF-binding protein-3 in normal and malignant colon
and its influence on apoptosis.
Endocr Relat Cancer. 2005 Dec;12(4):891-901.
PMID: 16322329 [PubMed - indexed for MEDLINE]
Banerjea A, Feakins RM, Nickols CD, Phillips SM, Powar MP, Bustin SA, Dorudi
S.
Immunogenic hsp-70 is overexpressed in colorectal cancers with high-degree
microsatellite instability.
Dis Colon Rectum. 2005 Dec;48(12):2322-8.
PMID: 16258706 [PubMed - indexed for MEDLINE]
Ahmed S, Banerjea A, Hands RE, Bustin S, Dorudi S.
Microarray profiling of colorectal cancer in Bangladeshi patients.
Colorectal Dis. 2005 Nov;7(6):571-5.
PMID: 16232237 [PubMed - indexed for MEDLINE]
Bustin SA, Mueller R.
Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical
diagnosis.
Clin Sci (Lond). 2005 Oct;109(4):365-79. Review.
PMID: 16171460 [PubMed - indexed for MEDLINE]
Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GA, Zumla
A.
The implications of using an inappropriate reference gene for real-time reverse
transcription PCR data normalization.
Anal Biochem. 2005 Sep 1;344(1):141-3. No abstract available.
PMID: 16054107 [PubMed - indexed for MEDLINE]
Bustin SA.
Real-time, fluorescence-based quantitative PCR: a snapshot of current
procedures and preferences.
Expert Rev Mol Diagn. 2005 Jul;5(4):493-8.
PMID: 16013967 [PubMed - in process]
Bustin SA, Benes V, Nolan T, Pfaffl MW.
Quantitative real-time RT-PCR--a perspective.
J Mol Endocrinol. 2005 Jun;34(3):597-601. Review.
PMID: 15956331 [PubMed - indexed for MEDLINE]
Banerjea A, Bustin SA, Dorudi S.
The immunogenicity of colorectal cancers with high-degree microsatellite
instability.
World J Surg Oncol. 2005 May 12;3:26.
PMID: 15890075 [PubMed]
Huggett J, Dheda K, Bustin S, Zumla A.
Real-time RT-PCR normalisation; strategies and considerations.
Genes Immun. 2005 Jun;6(4):279-84. Review.
PMID: 15815687 [PubMed - indexed for MEDLINE]
Bustin SA, Dorudi S.
Gene expression profiling for molecular staging and prognosis prediction in
colorectal cancer.
Expert Rev Mol Diagn. 2004 Sep;4(5):599-607. Review.
PMID: 15347254 [PubMed - indexed for MEDLINE]
Jenkins PJ, Bustin SA.
Evidence for a link between IGF-I and cancer.
Eur J Endocrinol. 2004 Aug;151 Suppl 1:S17-22. Review.
PMID: 15339239 [PubMed - indexed for MEDLINE]
Bustin SA, Nolan T.
Pitfalls of quantitative real-time reverse-transcription polymerase chain
reaction.
J Biomol Tech. 2004 Sep;15(3):155-66. Review.
PMID: 15331581 [PubMed - indexed for MEDLINE]
Banerjea A, Ahmed S, Hands RE, Huang F, Han X, Shaw PM, Feakins R, Bustin
SA, Dorudi S.
Colorectal cancers with microsatellite instability display mRNA expression
signatures characteristic of increased immunogenicity.
Mol Cancer. 2004 Aug 6;3:21.
PMID: 15298707 [PubMed - indexed for MEDLINE]
Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A.
Validation of housekeeping genes for normalizing RNA expression in real-time
PCR.
Biotechniques. 2004 Jul;37(1):112-4, 116, 118-9.
PMID: 15283208 [PubMed - indexed for MEDLINE]
Sanderson IR, Bustin SA, Dziennis S, Paraszczuk J, Stamm DS.
Age and diet act through distinct isoforms of the class II transactivator gene
in mouse intestinal epithelium.
Gastroenterology. 2004 Jul;127(1):203-12.
PMID: 15236186 [PubMed - indexed for MEDLINE]
Li SR, Ng CF, Banerjea A, Ahmed S, Hands R, Powar M, Ogunkolade W, Dorudi
S, Bustin SA.
Differential expression patterns of the insulin-like growth factor 2 gene in
human colorectal cancer.
Tumour Biol. 2004 Jan-Apr;25(1-2):62-8.
PMID: 15192314 [PubMed - indexed for MEDLINE]
22: Phillips SM, Banerjea A, Feakins R, Li SR, Bustin SA, Dorudi S.
Tumour-infiltrating lymphocytes in colorectal cancer with microsatellite
instability are activated and cytotoxic.
Br J Surg. 2004 Apr;91(4):469-75.
PMID: 15048750 [PubMed - indexed for MEDLINE]
23: Banerjea A, Phillips SM, Dorudi S, Bustin SA.
Colorectal cancers with mononucleotide microsatellite instability can be
identified using microfabricated chip technology.
Anal Biochem. 2003 Nov 1;322(1):130-3. No abstract available.
PMID: 14705790 [PubMed - indexed for MEDLINE]
24: Bustin SA, Siddiqi S, Ahmed S, Hands R, Dorudi S.
Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase
C mRNA levels in lymph nodes may not predict treatment failure in colorectal
cancer patients.
Int J Cancer. 2004 Jan 20;108(3):412-7.
PMID: 14648708 [PubMed - indexed for MEDLINE]
25: Vithana EN, Abu-Safieh L, Pelosini L, Winchester E, Hornan D, Bird AC, Hunt
DM, Bustin SA, Bhattacharya SS.
Expression of PRPF31 mRNA in patients with autosomal dominant retinitis
pigmentosa: a molecular clue for incomplete penetrance?
Invest Ophthalmol Vis Sci. 2003 Oct;44(10):4204-9.
PMID: 14507862 [PubMed - indexed for MEDLINE]
26: Kola B, Korbonits M, Diaz-Cano S, Kaltsas G, Morris DG, Jordan S, Metherell
L, Powell M, Czirjak S, Arnaldi G, Bustin S, Boscaro M, Mantero F, Grossman AB.
Reduced expression of the growth hormone and type 1 insulin-like growth factor
receptors in human somatotroph tumours and an analysis of possible mutations of
the growth hormone receptor.
Clin Endocrinol (Oxf). 2003 Sep;59(3):328-38.
PMID: 12919156 [PubMed - indexed for MEDLINE]
27: Li SR, Dorudi S, Bustin SA.
Identification of differentially expressed genes associated with colorectal
cancer liver metastasis.
Eur Surg Res. 2003 Jul-Aug;35(4):327-36.
PMID: 12802093 [PubMed - indexed for MEDLINE]
28: Bustin SA, Gyselman VG, Siddiqi S, Dorudi S.
Cytokeratin 20 is not a tissue-specific marker for the detection of malignant
epithelial cells in the blood of colorectal cancer patients.
Int J Surg Investig. 2000;2(1):49-57.
PMID: 12774338 [PubMed - indexed for MEDLINE]
29: Kirkpatrick KL, Ogunkolade W, Elkak AE, Bustin S, Jenkins P, Ghilchick M,
Newbold RF, Mokbel K.
hTERT expression in human breast cancer and non-cancerous breast tissue:
correlation with tumour stage and c-Myc expression.
Breast Cancer Res Treat. 2003 Feb;77(3):277-84.
PMID: 12602927 [PubMed - indexed for MEDLINE]
30: Laban C, Bustin SA, Jenkins PJ.
The GH-IGF-I axis and breast cancer.
Trends Endocrinol Metab. 2003 Jan;14(1):28-34. Review.
PMID: 12475609 [PubMed - indexed for MEDLINE]
31: Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, Pazzagli M,
Bustin SA, Orlando C.
Quantitative real-time reverse transcription polymerase chain reaction:
normalization to rRNA or single housekeeping genes is inappropriate for human
tissue biopsies.
Anal Biochem. 2002 Oct 15;309(2):293-300.
PMID: 12413463 [PubMed - indexed for MEDLINE]
32: Bustin SA.
Tenth annual nucleic acid-based technologies: time to stop and think.
Washington, DC, USA, 24-26 June, 2002.
Expert Rev Mol Diagn. 2002 Sep;2(5):405-8. No abstract available.
PMID: 12271812 [PubMed - indexed for MEDLINE]
33: Bustin SA, Dorudi S, Phillips SM, Feakins RM, Jenkins PJ.
Local expression of insulin-like growth factor-I affects angiogenesis in
colorectal cancer.
Tumour Biol. 2002 May-Jun;23(3):130-8.
PMID: 12218293 [PubMed - indexed for MEDLINE]
34: Kirkpatrick K, Ogunkolade W, Elkak A, Bustin S, Jenkins P, Ghilchik M,
Mokbel K.
The mRNA expression of cyclo-oxygenase-2 (COX-2) and vascular endothelial
growth factor (VEGF) in human breast cancer.
Curr Med Res Opin. 2002;18(4):237-41.
PMID: 12201625 [PubMed - indexed for MEDLINE]
35: Bustin SA.
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR):
trends and problems.
J Mol Endocrinol. 2002 Aug;29(1):23-39. Review.
PMID: 12200227 [PubMed - indexed for MEDLINE]
36: Ogunkolade BW, Boucher BJ, Prahl JM, Bustin SA, Burrin JM, Noonan K, North
BV, Mannan N, McDermott MF, DeLuca HF, Hitman GA.
Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians.
Diabetes. 2002 Jul;51(7):2294-300.
PMID: 12086963 [PubMed - indexed for MEDLINE]
37: Bustin SA, Dorudi S.
The value of microarray techniques for quantitative gene profiling in molecular diagnostics.
Trends Mol Med. 2002 Jun;8(6):269-72.
PMID: 12067612 [PubMed - indexed for MEDLINE]
38: Gnanapavan S, Kola B, Bustin SA, Morris DG, McGee P, Fairclough P,
Bhattacharya S, Carpenter R, Grossman AB, Korbonits M.
The tissue distribution of the mRNA of ghrelin and subtypes of its receptor,
GHS-R, in humans.
J Clin Endocrinol Metab. 2002 Jun;87(6):2988.
PMID: 12050285 [PubMed - indexed for MEDLINE]
39: Ogunkolade BW, Boucher BJ, Fairclough PD, Hitman GA, Dorudi S, Jenkins PJ,
Bustin SA.
Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase mRNA in individuals with
colorectal cancer.
Lancet. 2002 May 25;359(9320):1831-2.
PMID: 12044381 [PubMed - indexed for MEDLINE]
40: Bustin SA, Jenkins PJ.
The growth hormone-insulin-like growth factor-I axis and colorectal cancer.
Trends Mol Med. 2001 Oct;7(10):447-54. Review.
PMID: 11597519 [PubMed - indexed for MEDLINE]
41: Bustin SA, Li SR, Phillips S, Dorudi S.
Expression of HLA class II in colorectal cancer: evidence for enhanced
immunogenicity of microsatellite-instability-positive tumours.
Tumour Biol. 2001 Sep-Oct;22(5):294-8.
PMID: 11553859 [PubMed - indexed for MEDLINE]
42: Bustin SA, Li SR, Dorudi S.
Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is
downregulated in human colorectal cancer.
DNA Cell Biol. 2001 Jun;20(6):331-8.
PMID: 11445004 [PubMed - indexed for MEDLINE]
43: Bustin S.
Protein shuttles, IGF-I and colorectal cancer.
Trends Mol Med. 2001 Jan;7(1):9. No abstract available.
PMID: 11427994 [PubMed - indexed for MEDLINE]
44: Miraki-Moud F, Jenkins PJ, Fairclough PD, Jordan S, Bustin SA, Jones AM,
Lowe DG, Monson JP, Grossman AB, Besser GM, Camacho-Hubner C.
Increased levels of insulin-like growth factor binding protein-2 in sera and
tumours from patients with colonic neoplasia with and without acromegaly.
Clin Endocrinol (Oxf). 2001 Apr;54(4):499-508.
PMID: 11318786 [PubMed - indexed for MEDLINE]
45: Van Trappen PO, Gyselman VG, Lowe DG, Ryan A, Oram DH, Bosze P, Weekes AR,
Shepherd JH, Dorudi S, Bustin SA, Jacobs IJ.
Molecular quantification and mapping of lymph-node micrometastases in cervical
cancer.
Lancet. 2001 Jan 6;357(9249):15-20.
PMID: 11197354 [PubMed - indexed for MEDLINE]
46: Korbonits M, Bustin SA, Kojima M, Jordan S, Adams EF, Lowe DG, Kangawa K,
Grossman AB.
The expression of the growth hormone secretagogue receptor ligand ghrelin in
normal and abnormal human pituitary and other neuroendocrine tumors.
J Clin Endocrinol Metab. 2001 Feb;86(2):881-7.
PMID: 11158061 [PubMed - indexed for MEDLINE]
47: Bustin SA.
Chemoprevention of intestinal neoplasia
Mol Med Today. 2000 Dec;6(12):459. No abstract available.
PMID: 11099948 [PubMed - as supplied by publisher]
48: Bustin SA.
Absolute quantification of mRNA using real-time reverse transcription
polymerase chain reaction assays.
J Mol Endocrinol. 2000 Oct;25(2):169-93. Review.
PMID: 11013345 [PubMed - indexed for MEDLINE]
49: Bustin SA.
Nurture leads the race in the control of cancer.
Mol Med Today. 2000 Oct;6(10):380. No abstract available.
PMID: 11006522 [PubMed - indexed for MEDLINE]
50: Bustin SA.
'Lymphochips' and cancer profiles.
Mol Med Today. 2000 Jun;6(6):218. No abstract available.
PMID: 10939839 [PubMed - indexed for MEDLINE]
51: Li SR, Gyselman VG, Dorudi S, Bustin SA.
Elevated levels of RanBP7 mRNA in colorectal carcinoma are associated with
increased proliferation and are similar to the transcription pattern of the
proto-oncogene c-myc.
Biochem Biophys Res Commun. 2000 May 10;271(2):537-43.
PMID: 10799331 [PubMed - indexed for MEDLINE]
52: Li SR, Gyselman VG, Lalude O, Dorudi S, Bustin SA.
Transcription of the inositol polyphosphate 1-phosphatase gene (INPP1) is
upregulated in human colorectal cancer.
Mol Carcinog. 2000 Apr;27(4):322-9.
PMID: 10747296 [PubMed - indexed for MEDLINE]
Book chapters
McKay, IA, Bustin, SA, Brown, KD (1998) Engineered growth factors and receptors, and their applications. In: Growth Factors and Receptors-a practical approach. pp 1-17. I.A. McKay and K.D. Brown, Eds. Oxford University Press, Oxford, U.K.
Bustin, SA (2002) Real-time reverse transcription polymerase chain reaction. In: Analysing Gene expression A Handbook of Methods: Possibilities and Pitfalls pp-345-358. S. Lorkowski and P. Cullen, Eds Wiley-VCH, Weinheim
Bustin, SA (2004) Meaningful Quantification of mRNA using real-time PCR. pp 225-233 In: PCR technology-current innovations (2nd Edition) HG Griffin, A Griffin Eds, CRC press, Boca Raton, Fl
Bustin, SA and Nolan T (2004) Analysis of mRNA expression by real-time PCR. pp 125-184 In: Methods in Real-time PCR. Saunders N, Logan L, Edwards K (Eds) Horizon Bioscience Wymondham, UK
Bustin SA (2004) Quantification of nucleic acids by PCR. pp 5-46. In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Good Laboratory Practice. pp 123-139. In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Template Handling, preparation and quantification. pp141--213. In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Chemistries. pp 215-278 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Primers and probes. pp 279-327 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Instrumentation. pp 329-357 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Basic RT-PCR considerations pp 359-395 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) The RT step pp 397-438 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) Data analysis and interpretation pp 439-491 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Nolan T (2004) The qPCR does not work? pp 493-524 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Dorudi S (2004) Detection of “tissue-specific” mRNA in the blood and lymph nodes of patient without colorectal cancer pp 815-832 In: An A-Z manual of quantitative PCR, IUL Press, La Jolla, Ca USA.
Bustin, SA and Jenkins, PJ (2005) Colorectal cancer and the growth hormone/insulin-like growth factor-I axis. pp 83-114 In: Advances in Colorectal Cancer Nova Science Publishers, Inc. Hauppauge NY
Bustin, SA (2005) Real-time reverse transcription PCR. pp 1131-1135. In: Encyclopedia of Diagnostic Genomics and Proteomics. (J Fuchs and M Podda, Eds) Marcel Dekker Inc New York
Bustin, SA (2005) Real-time PCR. pp 1117-1125. In: Encyclopedia of Diagnostic Genomics and Proteomics. (J Fuchs and M Podda, Eds) Marcel Dekker Inc New York
Huggett, J, Dheda, K. Bustin, SA (2006) Normalisation in: Real-time PCR. T Dorak (Ed), Taylor and Francis, Abingdon, UK
