The qPCR "Bible"

This is not just a cook book for real-time quantitative PCR (qPCR). Admittedly, there are lots of recipes from distinguished contributors and I have attempted to collect, sift through and rationalise the vast amount of information that is available on this subject. And yes, this book was conceived as a comprehensive hands-on manual to allow both the novice researcher and the expert to set up and carry out qPCR assays from scratch. However, this book also sets out to explain as many features of qPCR as possible, provide alternative viewpoints and methods and, perhaps most importantly, aims to stimulate the researcher into generating, interpreting and publishing data that are reproducible, reliable, and biologically meaningful.

The very nature of the technology means that new chemistries, protocols, and instruments come and go. Any book would struggle to keep up-to-date with such developments. However, by emphasising and describing the very basic steps that must be right and providing step-by-step guidance on how to achieve reproducible results and interpret them correctly, this book will remain topical. My hope is that this book will contribute to taking quantitative PCR forward to a new stage of use as a standard, reliable, and useful molecular technique.

Available from www.amazon.com (click on book)